pAAV-CamKII-GFP-P2A-bReaches-minWPRE
(Plasmid #118276)

• Purpose: Expresses EGFP and bReaChES under a minimal CamKII Promoter
• Depositing Lab: Dietmar Schmitz
• Publication: Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8.

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Backbone

• Vector backbone: CW3SL
• Backbone manufacturer: Bong-Kiun Kaang
• Backbone size w/o insert (bp): 3771
• Total vector size (bp): 5591
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: EGFP
• Insert Size (bp): 710
• GenBank ID:
• Promoter: CamK II
• Tag / Fusion Protein:
  • P2A (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GTTCTCCGTTTGCACTCAGGA
• 3′ sequencing primer: GGGTTCTCCTCCACGTCTC

Gene/Insert 2

• Gene/Insert name: bReaCHES
• Species: Synthetic
• Insert Size (bp): 915
• GenBank ID:
• Promoter: CamK II

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CGGAGCTACTAACTTCAGCC
• 3′ sequencing primer: CGTATCCACATAGCGTAAAAGGAGCAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry