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Addgene

pAAV-DYRK1A-2A-mCherry
(Plasmid #118272)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118272 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-2A-mCherry
  • Backbone size w/o insert (bp) 5394
  • Total vector size (bp) 7656
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a
  • Alt name
    DYRK1A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2262
  • GenBank ID
    NM_001347731.1
  • Entrez Gene
    Dyrk1a (a.k.a. 2310043O08Rik, D16Ertd272e, D16Ertd493e, Dyrk, Gm10783, Mnbh, Mp86, mmb)
  • Promoter CMV with beta global intron
  • Tags / Fusion Proteins
    • 2A peptide (C terminal on insert)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer tggttgggataaggctggat
  • 3′ sequencing primer GFP reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-DYRK1A-2A-mCherry was a gift from Vance Lemmon (Addgene plasmid # 118272 ; http://n2t.net/addgene:118272 ; RRID:Addgene_118272)
  • For your References section:

    Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology. Slepak TI, Salay LD, Lemmon VP, Bixby JL. Cytoskeleton (Hoboken). 2012 Jul;69(7):514-27. doi: 10.1002/cm.21021. Epub 2012 Mar 7. 10.1002/cm.21021 PubMed 22359282