N229A Lo-MetQ
(Plasmid #118268)

• Purpose: N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
• Depositing Lab: Douglas Rees
• Publication: Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. pii: 1811003115. doi: 10.1073/pnas.1811003115.

Backbone

• Vector backbone: pET19b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5652
• Total vector size (bp): 6461
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MetQ (N229A)
• Alt name: Methionine Binding Protein
• Species: E. coli
• Insert Size (bp): 747
• Mutation: Changed Asparagine (Asn) 229 to Alanine (Ala)
• GenBank ID: 944893
• Entrez Gene: metQ (a.k.a. b0197, ECK0197, metD, yaeC)
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 10x His Tag (N terminal on insert)
  • Enterokinase Cleavage Site (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • nalo-metq-in-pet19b.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry