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Addgene

pBSV2_PflaB-mCherryBb
(Plasmid #118243)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118243 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBSV2_2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flagellin promoter driven mCherry
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F(-21)
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSV2_PflaB-mCherryBb was a gift from Christine Jacobs-Wagner (Addgene plasmid # 118243 ; http://n2t.net/addgene:118243 ; RRID:Addgene_118243)
  • For your References section:

    Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi. Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C. Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 10.1128/AEM.01824-18. 10.1128/AEM.01824-18 PubMed 30315081