pBSV2G_PflaB-msfCFPBb
(Plasmid
#118232)
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PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSV2G_2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameflagellin promoter driven msfCFP
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F(-21) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSV2G_PflaB-msfCFPBb was a gift from Christine Jacobs-Wagner (Addgene plasmid # 118232 ; http://n2t.net/addgene:118232 ; RRID:Addgene_118232) -
For your References section:
Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi. Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C. Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 10.1128/AEM.01824-18. 10.1128/AEM.01824-18 PubMed 30315081