Skip to main content
Addgene

pCBEclos-cbei2385-g2-opt
(Plasmid #118215)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pXY1
  • Backbone manufacturer
    Sheng Yang's lab
  • Backbone size w/o insert (bp) 5577
  • Total vector size (bp) 10690
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Apobec1, Casp nickase, UG1-sgRNA
  • Species
    Clostridium
  • Insert Size (bp)
    5113
  • Promoter Ptm

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer aaataaattaagtatata
  • 3′ sequencing primer ggatgtgctgcaaggcga
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBEclos-cbei2385-g2-opt was a gift from Sheng Yang (Addgene plasmid # 118215 ; http://n2t.net/addgene:118215 ; RRID:Addgene_118215)

  • For your References section:

    CRISPR-Cas9(D10A) nickase-assisted base editing in the solvent producer Clostridium beijerinckii. Li Q, Seys FM, Minton NP, Yang J, Jiang Y, Jiang W, Yang S. Biotechnol Bioeng. 2019 Feb 10. doi: 10.1002/bit.26949. 10.1002/bit.26949 PubMed 30739328
Related items:
Commonly requested with: