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Addgene

FLAG-OTUB1 T134R-pcDNA3.1
(Plasmid #118211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 6253
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OTUB1
  • Alt name
    Otubain-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    changed threonine 134 to arginine
  • Entrez Gene
    OTUB1 (a.k.a. HSPC263, OTB1, OTU1)
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-OTUB1 T134R-pcDNA3.1 was a gift from Cynthia Wolberger (Addgene plasmid # 118211 ; http://n2t.net/addgene:118211 ; RRID:Addgene_118211)

  • For your References section:

    OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination. Pasupala N, Morrow ME, Que LT, Malynn BA, Ma A, Wolberger C. J Biol Chem. 2018 Nov 23;293(47):18285-18295. doi: 10.1074/jbc.RA118.004677. Epub 2018 Oct 3. 10.1074/jbc.RA118.004677 PubMed 30282802
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