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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1181 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep426 GPD
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehtt 25Q
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SpeciesH. sapiens (human)
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Insert Size (bp)250
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GenBank IDNM_002111
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N terminal region of Ht with polyQ repeat length of 25 fused to GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p426 25Q GPD was a gift from Susan Lindquist (Addgene plasmid # 1181 ; http://n2t.net/addgene:1181 ; RRID:Addgene_1181) -
For your References section:
Aggregation of huntingtin in yeast varies with the length of the polyglutamine expansion and the expression of chaperone proteins. Krobitsch S, Lindquist S. Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94. 10.1073/pnas.97.4.1589 PubMed 10677504