HsSPHK1
(Plasmid #118091)

• Purpose: Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
• Depositing Lab: Kevin Lynch
• Publication: Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. doi: 10.1371/journal.pone.0192179. eCollection 2018.

Backbone

• Vector backbone: pYES2
• Backbone size w/o insert (bp): 5800
• Total vector size (bp): 6974
• Modifications to backbone: Codon optimized for S. cerevisiae expression
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens Sphingosine Kinase 1
• Alt name: HsSPHK1
• Species: H. sapiens (human)
• Insert Size (bp): 1158
• GenBank ID: KY859176.1
• Promoter: GAL1, 10
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry