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MLRV
(Plasmid #118069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1 vector Alpha2
  • Backbone manufacturer
    Self made; Derived from pBluescriptSK
  • Backbone size w/o insert (bp) 2327
  • Total vector size (bp) 13382
  • Modifications to backbone
    Kanamycin Resistance
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology ; Assembly of GB2.0 transcriptional units

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TB:5xNF-κβ:RedF:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2205
  • Promoter 5xNF-KB::miniP

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer cgctGTCAggagGGGAATTTC
  • 3′ sequencing primer AAGCTCACATCTTGGCCACGGGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TB:4xTGF-β:FLuc:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2111
  • Promoter 4xTGF-B::miniP

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer TTCTCTcgctGTCAGGAGG
  • 3′ sequencing primer TAGAAGGCACAGAAGCttacacggcga
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TB:5xE-box:Renilla:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1477
  • Promoter 5xEbox::miniP

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer ATTTCTCTcgctGTCAGGAGCACGTGTGC
  • 3′ sequencing primer GAACAGTGAGCTTCTGTGCCTTCTAGTTGCCAGC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    TB:2xp53:NLuc:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1076
  • Promoter 2xp53::miniP

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer ATTTCTCTcgctGTCAGGAGTACAGAAC
  • 3′ sequencing primer CCTGAGCTTCTGTGCCTTCTAGTTGCCAGCCATCT
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    TB:6xAP-1:GrRenilla:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1497
  • Promoter 6xAP1::miniP

Cloning Information for Gene/Insert 5

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer ATTTCTCTcgctGTCAGGAGTGAGT
  • 3′ sequencing primer GTGAGGCTTCTGTGCCTTCTAGTTGCCAGCCAT
    • (Common Sequencing Primers)

Gene/Insert 6

  • Gene/Insert name
    CMV:ELuc:bGHpA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2449
  • Promoter CMV

Cloning Information for Gene/Insert 6

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBi (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer CAGGAGTAGTTATTAATAGTAATCAATTACG
  • 3′ sequencing primer CGGCTTGAGCTTCTGTGCCTTCTAGTTGCCAGCCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert can be released with BsmBI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLRV was a gift from Koen Venken (Addgene plasmid # 118069 ; http://n2t.net/addgene:118069 ; RRID:Addgene_118069)

  • For your References section:

    Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712
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