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Addgene

hCMV-IE1:GrRenilla
(Plasmid #118067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1 vector Alpha2
  • Backbone manufacturer
    Self made; Derived from pBluescriptSK
  • Backbone size w/o insert (bp) 2327
  • Total vector size (bp) 4097
  • Modifications to backbone
    Kanamycin Resistance
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology ; Assembly of GB2.0 transcriptional units

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV:GreenRenilla:bGHF
  • Alt name
    Cytomegalovirus enhancer and promoter driving the expression of the GrennRenillaLuciferase
  • Species
    Synthetic
  • Insert Size (bp)
    2517
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer aat gag ctg att taa caa aaa ttt aac gcg
  • 3′ sequencing primer ctt ttt acg gtt cct ggc ctt ttg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert can be released with BsmBI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCMV-IE1:GrRenilla was a gift from Koen Venken (Addgene plasmid # 118067 ; http://n2t.net/addgene:118067 ; RRID:Addgene_118067)

  • For your References section:

    Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712
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