hCMV-IE1:RedF
(Plasmid #118064)

• Purpose: Transcriptional unit for constitutive expression of RedF in mammalian cells
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: ColE1 vector Alpha2
• Backbone manufacturer: Self made; Derived from pBluescriptSK
• Backbone size w/o insert (bp): 2327
• Total vector size (bp): 4808
• Modifications to backbone: Kanamycin Resistance
• Vector type: Mammalian Expression, Luciferase, Synthetic Biology ; Assembly of GB2.0 transcriptional units

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV:RedF:bGHF
• Alt name: Cytomegalovirus enhancer and promoter driving the expression of the RedF Luciferase
• Species: Synthetic
• Insert Size (bp): 2517
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: aat gag ctg att taa caa aaa ttt aac gcg
• 3′ sequencing primer: ctt ttt acg gtt cct ggc ctt ttg

Resource Information

• Supplemental Documents:
  • Alfa2 CMV_RedF.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert can be released with BsmBI