pNLuc
(Plasmid #118058)

• Purpose: GBPart - NLuc Luciferase CDS
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: pUPD3
• Backbone manufacturer: Self made; Derived from pBluescriptSK
• Backbone size w/o insert (bp): 2343
• Total vector size (bp): 2680
• Modifications to backbone: Chloramphenicol Resistance
• Vector type: Luciferase, Synthetic Biology ; GBPart

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nano Luciferase
• Alt name: NanoLuc
• Alt name: NLuc
• Species: Synthetic; Directed evolved synthetic, from Oplophorus gracilirostris. Promega.
• Insert Size (bp): 517

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: tctatagtgtcacctaaat
• 3′ sequencing primer: taatacgactcactatagg

Resource Information

• Supplemental Documents:
  • pNLuc.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert can be released with BsaI