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Addgene

pRedF
(Plasmid #118057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    Diego Orzaez Lab
  • Backbone size w/o insert (bp) 3057
  • Total vector size (bp) 4705
  • Modifications to backbone
    Ampicillin Resistance
  • Vector type
    Synthetic Biology ; Domestication of DNA parts for GoldenBraid asembly

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Red Firefly Luciferase CDS
  • Alt name
    Red mutant S286Y luciferase from Luciola curciata
  • Species
    Synthetic; Luciola curciata
  • Insert Size (bp)
    1684
  • Mutation
    Red mutant S286Y

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer gtcatagctgtttcctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert can be released with BsaI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRedF was a gift from Koen Venken (Addgene plasmid # 118057 ; http://n2t.net/addgene:118057 ; RRID:Addgene_118057)

  • For your References section:

    Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712
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