p6xAP-1_RE
(Plasmid
#118054)
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PurposeGBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUPD
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Backbone manufacturerDiego Orzaez Lab
- Backbone size w/o insert (bp) 3057
- Total vector size (bp) 3115
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6 copies of the AP-1 binding motif
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Alt name6xAP-1
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)58
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer gtcatagctgtttcctg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p6xAP-1_RE was a gift from Koen Venken (Addgene plasmid # 118054 ; http://n2t.net/addgene:118054 ; RRID:Addgene_118054) -
For your References section:
Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712