p5xE-Box
(Plasmid
#118052)
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PurposeGBPart - Operator/DNA Response element - 5 copies of the E-box motif
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118052 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUPD3
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Backbone manufacturerSelf made; Derived from pBluescriptSK
- Backbone size w/o insert (bp) 2343
- Total vector size (bp) 2385
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Modifications to backboneChloramphenicol Resistance
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Vector typeSynthetic Biology ; Domestication of DNA parts for GoldenBraid asembly
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5 copies of the E-box motif
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Alt namep5xE-Box
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)42
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer gtcatagctgtttcctg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5xE-Box was a gift from Koen Venken (Addgene plasmid # 118052 ; http://n2t.net/addgene:118052 ; RRID:Addgene_118052) -
For your References section:
Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712