Alpha1
(Plasmid #118044)

• Purpose: (Empty Backbone) GoldenBraid2.0 Alpha 1 destination vector
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.

Backbone

• Vector backbone: ColE1 vector Alpha1
• Backbone manufacturer: Self made; Derived from pBluescriptSK
• Backbone size (bp): 2326
• Modifications to backbone: Kanamycin Resistance; GB2.0 Alpha1 cassette
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression, Synthetic Biology ; Assembly of GB2.0 transcriptional units

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: aat gag ctg att taa caa aaa ttt aac gcg
• 3′ sequencing primer: ctt ttt acg gtt cct ggc ctt ttg

Resource Information

• Supplemental Documents:
  • Alpha1.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a BsaI GB2.0 Alpha entry vector. Any assembled unit(s) can be released with BsmBI for further cloning into Omega level vectors.