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Addgene

pUPD3
(Plasmid #118043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUPD
  • Backbone manufacturer
    Self made; Derived from pBluescriptSK
  • Backbone size (bp) 2804
  • Modifications to backbone
    Chloramphenicol Resistance
  • Vector type
    Synthetic Biology ; Domestication of DNA parts for GoldenBraid asembly

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer tctatagtgtcacctaaat
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a GB2.0 domesticator vector. GBParts can be assembled in a BsmBI-ligation reaction. Any assembled Part can be released with BsaI for further cloning into Alpha level vectors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUPD3 was a gift from Koen Venken (Addgene plasmid # 118043 ; http://n2t.net/addgene:118043 ; RRID:Addgene_118043)

  • For your References section:

    Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying. Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young DW, Venken KJT. Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y. 10.1038/s41467-019-13651-y PubMed 31836712
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