Myc-gamma2s-EGFP
(Plasmid #118040)

• Purpose: GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus
• Depositing Lab: Angel de Blas
• Publication: GABAA receptor subunit plasmids (unpublished)

Backbone

• Vector backbone: pEGFP N1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRG2
• Species: R. norvegicus (rat)
• Entrez Gene: Gabrg2
• Tags / Fusion Proteins:
  • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA originally cloned in Peter Seeburg’s lab in Heidelberg.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry