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Addgene

Myc-delta
(Plasmid #118037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 118037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GABRD
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gabrd (a.k.a. GABAA-RD)
  • Tag / Fusion Protein
    • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The rat delta was originally from Dr. Robert Macdonald at Vanderbilt.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-delta was a gift from Angel de Blas (Addgene plasmid # 118037 ; http://n2t.net/addgene:118037 ; RRID:Addgene_118037)

  • For your References section:

    Clustered and non-clustered GABAA receptors in cultured hippocampal neurons. Christie SB, Li RW, Miralles CP, Yang B-, De Blas AL. Mol Cell Neurosci. 2006 Jan;31(1):1-14. doi: 10.1016/j.mcn.2005.08.014. Epub 2005 Sep 21. 10.1016/j.mcn.2005.08.014 PubMed 16181787

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