Myc-gamma2s
(Plasmid
#118036)
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PurposeGABAA receptor expression (gamma2 short subunit) Myc-tag near N-terminus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGABRG2
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SpeciesR. norvegicus (rat)
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Entrez GeneGabrg2
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Tag
/ Fusion Protein
- cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA originally cloned in Peter Seeburg’s lab in Heidelberg.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc-gamma2s was a gift from Angel de Blas (Addgene plasmid # 118036 ; http://n2t.net/addgene:118036 ; RRID:Addgene_118036) -
For your References section:
Clustered and non-clustered GABAA receptors in cultured hippocampal neurons. Christie SB, Li RW, Miralles CP, Yang B-, De Blas AL. Mol Cell Neurosci. 2006 Jan;31(1):1-14. doi: 10.1016/j.mcn.2005.08.014. Epub 2005 Sep 21. 10.1016/j.mcn.2005.08.014 PubMed 16181787