hSyn1-2xNLS-mTurquoise2
(Plasmid #118025)

• Purpose: An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4561
• Total vector size (bp): 5339
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 2xNLS-mTurquoise2
• Species: Synthetic
• Insert Size (bp): 778
• Promoter: hSyn1
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: CCAAAGAAGAAGCGTAAGGTAgg (unknown if destroyed)
• 3′ cloning site: GCCCCAAGAAaAAGCGgAAGGTgTG (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry