Six5 pGWB402
(Plasmid
#118013)
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Purposeexpresses Six5 (without signal peptide). Used in combination with Avr2-GFP pZK538(#118011) for visualization of cell-to-cell movement of Avr2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 118013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB402
- Total vector size (bp) 10330
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Vector typeBacterial Expression, Plant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedspSix5
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SpeciesFusarium oxysporum f.sp lycopersici
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Insert Size (bp)306
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GenBank IDACN87967.1
- Promoter 35S
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Six5 pGWB402 was a gift from FLW Takken (Addgene plasmid # 118013 ; http://n2t.net/addgene:118013 ; RRID:Addgene_118013) -
For your References section:
Visualization and Quantification of Cell-to-cell Movement of Proteins in Nicotiana benthamiana. Blekemolen, M. C., Tark-Dame, M. and Takken, F. L.. Bio-protocol 8(24): e3114. 10.21769/BioProtoc.3114 PubMed 29481865