NSm-GFP pZK538
(Plasmid #118012)

• Purpose: expresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).
• Depositing Labs: FLW Takken, Xiaorong Tao
• Publication: et al Bio-protocol 8(24): e3114.

Backbone

• Vector backbone: pZK538
• Total vector size (bp): 14169
• Vector type: Bacterial Expression, Plant Expression
• Selectable markers: Phosphinothricin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NSm
• Species: Tomato spotted wilt virus
• Insert Size (bp): 909
• GenBank ID: JF960236.1
• Promoter: 35S
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Caccatttacgaacgatagcc

Gene/Insert 2

• Gene/Insert name: mCherry
• Insert Size (bp): 711
• Promoter: 35S
• Tag / Fusion Protein:
  • HDEL (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: mCherry-R

Resource Information

• Supplemental Documents:
  • pZK538.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry