pcDNA3.1-EPG
(Plasmid #117978)

• Purpose: Express EPG in mamalian cells
• Depositing Lab: Assaf Gilad
• Publication: Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1038/s41598-018-27087-9.

Backbone

• Vector backbone: pcDNA 3.1 V5 HIS
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 5900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Electromagnetic perceptive gene
• Alt name: EPG
• Species: Kryptopterus bicirrhis
• Insert Size (bp): 399
• GenBank ID: MH590650.1 MH590650.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • v5 (C terminal on backbone)
  • HIS (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: V5 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was originally created by Dr. Gilad at Johns Hopkins University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry