JS200 strain
(Bacterial strain #11794)

• Depositing Lab: Lawrence Loeb
• Publication: Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 100(17):9727-32.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: grow at 30˚C, and store when it is in exponential phase (do not allow it to get too dense).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JS200
• Alt name: polA recA718

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For use with pEP PolI (addgene #11722) and pWT PolI (addgene #11721). This strain contains a temp sens mutation in PolI.