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Addgene

pmGFP-ADAR2
(Plasmid #117929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADAR2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001112 NM_001112
  • Entrez Gene
    ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, NEDHYMS, RED1)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AAAGGGgaattcATGGATATAGAAGATGAAGAAAACATG
  • 3′ sequencing primer AAAAGGAAAAgcggccgcTCAGGGCGTGAGTGAGAACTGGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmGFP-ADAR2 was a gift from Kumiko Ui-Tei (Addgene plasmid # 117929 ; http://n2t.net/addgene:117929 ; RRID:Addgene_117929)

  • For your References section:

    Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K. Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. 10.3390/genes8020068 PubMed 28208661
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