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PurposeADAR1-p110 expression plasmid.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameADAR1-p110
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SpeciesH. sapiens (human)
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GenBank IDNM_001193495 NM_001193495
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Entrez GeneADAR (a.k.a. ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136)
- Promoter CMV
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Tag
/ Fusion Protein
- myc-GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer AAAGGGaagcttATGGCCGAGATCAAGGAGAAAATC
- 3′ sequencing primer AAAGGGtctagaCTATACTGGGCAGAGATAAAAGTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmGFP-ADAR1-p110 was a gift from Kumiko Ui-Tei (Addgene plasmid # 117928 ; http://n2t.net/addgene:117928 ; RRID:Addgene_117928) -
For your References section:
Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K. Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. 10.3390/genes8020068 PubMed 28208661