pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
(Plasmid #117909)

• Purpose: Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM
• Depositing Lab: Kai Johnsson
• Publication: Yu et al Science. 2018 Sep 14;361(6407):1122-1126. doi: 10.1126/science.aat7992.

Backbone

• Vector backbone: pET51-b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5218
• Total vector size (bp): 6865
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
• Species: Synthetic
• Insert Size (bp): 1803
• Mutation: R98A on eDHFR
• Promoter: T7
• Tags / Fusion Proteins:
  • Strep-tag (N terminal on insert)
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: pET-RP

Resource Information

• Supplemental Documents:
  • pET51b(_)-NADPH-LUCID-2 (C50 29 nM).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry