pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM)
(Plasmid
#117908)
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PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 5.1 nM
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET51-b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5218
- Total vector size (bp) 6865
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSNAP_Pro30_[cpNLuc23_24]eDHFR
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SpeciesSynthetic
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Insert Size (bp)1803
- Promoter T7
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Tags
/ Fusion Proteins
- Strep-tag (N terminal on insert)
- His-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer pET-RP (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET51b(+)-NADPH-LUCID-1 (C50 5.1 nM) was a gift from Kai Johnsson (Addgene plasmid # 117908 ; http://n2t.net/addgene:117908 ; RRID:Addgene_117908) -
For your References section:
Semisynthetic sensor proteins enable metabolic assays at the point of care. Yu Q, Xue L, Hiblot J, Griss R, Fabritz S, Roux C, Binz PA, Haas D, Okun JG, Johnsson K. Science. 2018 Sep 14;361(6407):1122-1126. doi: 10.1126/science.aat7992. 10.1126/science.aat7992 PubMed 30213915
