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Addgene

pcDNA3.1-Clover-Prx2-mRuby2
(Plasmid #117906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5401
  • Total vector size (bp) 7420
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clover-Prx2-mRuby2
  • Alt name
    A pH-robust FRET sensor for hydrogen peroxide
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2019
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer gtcgaggctgatcagcg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Clover-Prx2-mRuby2 was a gift from Hadley Sikes (Addgene plasmid # 117906 ; http://n2t.net/addgene:117906 ; RRID:Addgene_117906)

  • For your References section:

    Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Langford TF, Huang BK, Lim JB, Moon SJ, Sikes HD. Nat Commun. 2018 Aug 7;9(1):3145. doi: 10.1038/s41467-018-05557-y. 10.1038/s41467-018-05557-y [pii] PubMed 30087344
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