-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 11790 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepREP4
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10200
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNFAT4
-
Alt nameNFAT4a isoform
-
SpeciesH. sapiens (human)
-
Entrez GeneNFATC3 (a.k.a. NF-AT4c, NFAT4, NFATX, n339260)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byHoey et al, 1995, PMID 7749981.
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid contains the NFAT4a isoform as described in the following publication: https://www.ncbi.nlm.nih.gov/pubmed/7749981 This isoform contains 708 amino acids.
Please note: Addgene NGS is unable to fully resolve the length of the poly-A tail in the 3'UTR
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pREP-NFAT4 was a gift from Anjana Rao (Addgene plasmid # 11790 ; http://n2t.net/addgene:11790 ; RRID:Addgene_11790)