pET15b CnA CnB
(Plasmid #11787)

• Depositing Lab: Anjana Rao
• Publication: Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-42.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CnA CnB
• Alt name: calcineurin
• Alt name: Ppp3ca (calcineurin A subunit alpha)
• Alt name: Ppp3r1 (calcineurin B, type I)
• Species: H. sapiens (human)
• Entrez Gene: PPP3R1 (a.k.a. CALNB1, CNB, CNB1)
• Entrez Gene: PPP3CA (a.k.a. ACCIID, CALN, CALNA, CALNA1, CCN1, CNA1, DEE91, IECEE, IECEE1, PPP2B)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • pET15b CnA CnB - Annotated file
• A portion of this plasmid was derived from a plasmid made by: JO Liu, 1997, PMID 9125515.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry