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pRSF-KpAGO207-1251
(Plasmid #117854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117854 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF Duet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Argonaute
  • Species
    Kluyveromyces polysporus
  • Insert Size (bp)
    3138
  • Mutation
    missing first 206 amino acid residues
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-SUMO encoded in vector (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer sumo forward
  • 3′ sequencing primer T7 terminator reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was cloned by Dr. Kotaro Nakanishi.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-KpAGO207-1251 was a gift from Kotaro Nakanishi & Dinshaw Patel (Addgene plasmid # 117854 ; http://n2t.net/addgene:117854 ; RRID:Addgene_117854)

  • For your References section:

    Structure of yeast Argonaute with guide RNA. Nakanishi K, Weinberg DE, Bartel DP, Patel DJ. Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. 10.1038/nature11211 PubMed 22722195
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