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pMEG_YLCas9_empty
(Plasmid #117826)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMEG_BB3_YL68N_AC
  • Backbone size w/o insert (bp) 4193
  • Vector type
    Bacterial Expression, Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Nourseothricin (clonNat), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YlpTEF_hCas9_YlCyc1TT
  • Insert Size (bp)
    5937

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (unknown if destroyed)
  • 3′ cloning site BbsI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMEG_YLCas9_empty was a gift from Michael Sauer (Addgene plasmid # 117826 ; http://n2t.net/addgene:117826 ; RRID:Addgene_117826)

  • For your References section:

    Golden Gate based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica. Egermeier M, Sauer M, Marx H. FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 10.1093/femsle/fnz022. 10.1093/femsle/fnz022 PubMed 30698703
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