BB1_23_YlGUT1
(Plasmid
#117823)
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PurposeEncodes glycerol kinase (GUT1) of Y. lipolytica for GoldenMOCS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBB1_L_23_syn_BsaI
- Backbone size w/o insert (bp) 1966
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYlGUT1
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Insert Size (bp)1517
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (unknown if destroyed)
- 3′ cloning site BbsI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BB1_23_YlGUT1 was a gift from Michael Sauer (Addgene plasmid # 117823 ; http://n2t.net/addgene:117823 ; RRID:Addgene_117823) -
For your References section:
Golden Gate based metabolic engineering strategy for wild-type strains of Yarrowia lipolytica. Egermeier M, Sauer M, Marx H. FEMS Microbiol Lett. 2019 Jan 29. pii: 5304171. doi: 10.1093/femsle/fnz022. 10.1093/femsle/fnz022 PubMed 30698703