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BioBrick pET-24a(+)
(Plasmid #117802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-24a(+)
  • Backbone size (bp) 5018
  • Modifications to backbone
    Modifications to backbone: polylinker changed to contain the four standard BioBrick restriction sites EcoRI, XbaI, SpeI and PstI; the remaining sequence lacks these 4 BioBrick sites. The 5’-to-3’ annotation of the insert is BglII, BioBrick prefix (EcoRI, NotI, XbaI), T7 RNA polymerase promoter, lac operator, epsilon translation enhancer, Shine-Dalgarno sequence, NdeI/ATG start codon, minigene (including XhoI site and VSV in vitro transcription terminator), stop codon, 119-nucleotide Tphi transcription terminator, BioBrick suffix (SpeI, NotI, PstI) and BspEI (not unique).
  • Vector type
    Escherichia coli

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BioBrick pET-24a(+) was a gift from Anthony Forster (Addgene plasmid # 117802 ; http://n2t.net/addgene:117802 ; RRID:Addgene_117802)

  • For your References section:

    Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase. Du L, Gao R, Forster AC. Biotechnol Bioeng. 2009 Dec 15;104(6):1189-96. doi: 10.1002/bit.22491. 10.1002/bit.22491 PubMed 19650080
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