pLD3 translation factors
(Plasmid
#117762)
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PurposeBioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBioBrick pET-24a(+)
- Backbone size w/o insert (bp) 5018
- Total vector size (bp) 23386
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Vector typeEscherichia coli
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Selectable markerstetracycline
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTranslation factor cistrons 22-30
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SpeciesEscherichia coli
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Insert Size (bp)18400
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication prior to proceeding.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD3 translation factors was a gift from Anthony Forster (Addgene plasmid # 117762 ; http://n2t.net/addgene:117762 ; RRID:Addgene_117762) -
For your References section:
De novo design and synthesis of a 30-cistron translation-factor module. Shepherd TR, Du L, Liljeruhm J, Samudyata, Wang J, Sjodin MOD, Wetterhall M, Yomo T, Forster AC. Nucleic Acids Res. 2017 Oct 13;45(18):10895-10905. doi: 10.1093/nar/gkx753. 10.1093/nar/gkx753 PubMed 28977654
