pLD1 translation factors
(Plasmid #117760)

• Purpose: BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli
• Depositing Lab: Anthony Forster
• Publication: Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10895-10905. doi: 10.1093/nar/gkx753.

Backbone

• Vector backbone: BioBrick pET-24a(+)
• Backbone size w/o insert (bp): 5018
• Total vector size (bp): 30152
• Vector type: Escherichia coli
• Selectable markers: tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Tetracyline and Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): BLR (F- ompT hsdSB(rB- mB-)gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR))
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Translation factor cistrons 1-13
• Species: Escherichia coli
• Insert Size (bp): 25100

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid can be prone to recombination. The depositing lab suggests selecting multiple clones for restriction digest analysis as described in the publication before proceeding.