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Depositing Lab
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Depositing OrganizationSanford Burnham Prebys Medical Discovery Institute
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 11776-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 11776-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCXN2
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Backbone manufacturerNiwa et al, Gene, v108, 1991
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevinculin
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SpeciesM. musculus (mouse)
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Insert Size (bp)3200
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Entrez GeneVcl (a.k.a. 9430097D22)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See author map (unknown if destroyed)
- 3′ cloning site See author map (unknown if destroyed)
- 5′ sequencing primer See author map
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 11776-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 11776-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCXN2-mVIN was a gift from Eileen Adamson (Addgene plasmid # 11776 ; http://n2t.net/addgene:11776 ; RRID:Addgene_11776) -
For your References section:
Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin. Xu W, Coll JL, Adamson ED. J Cell Sci. 1998 Jun;111 ( Pt 11):1535-44. doi: 10.1242/jcs.111.11.1535. 10.1242/jcs.111.11.1535 PubMed 9580561
Map uploaded by the depositor.
