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Addgene

pCarCBADE_ProB(I69E)A
(Plasmid #117756)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbaA5k
  • Backbone manufacturer
    Genebank
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
  • Alt name
    ProA
  • Alt name
    ProBI69E
  • Alt name
    CarCBADE
  • Species
    Pectobacterium carotovorum
  • Insert Size (bp)
    7011
  • GenBank ID
  • Promoter PLac(UV5)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTATAATGTGTGGAATTGTGAGC
  • 3′ sequencing primer AACGCCCTAGGTATAAACGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCarCBADE_ProB(I69E)A was a gift from Greg Bokinsky (Addgene plasmid # 117756 ; http://n2t.net/addgene:117756 ; RRID:Addgene_117756)

  • For your References section:

    Metabolic engineering of a carbapenem antibiotic synthesis pathway in Escherichia coli. Shomar H, Gontier S, van den Broek NJF, Tejeda Mora H, Noga MJ, Hagedoorn PL, Bokinsky G. Nat Chem Biol. 2018 Aug;14(8):794-800. doi: 10.1038/s41589-018-0084-6. Epub 2018 Jun 25. 10.1038/s41589-018-0084-6 PubMed 29942079
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