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pBS U6 WIP RNAi
(Plasmid #11774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS-U6-RNAi-dual-CMV-GFP
  • Backbone manufacturer
    Yang Shi's lab (Harvard Medical School)
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WIP RNAi
  • Alt name
    WASp Interacting Protein
  • Alt name
    WIP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    WIPF1 (a.k.a. PRPL-2, WAS2, WASPIP, WIP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 forward 20
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Yang Shi (Harvard Medical School) provided the pBS-U6 empty vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RNAi directed against this sequence in WIP: GGAGGTTTCCTGTGCCTTCT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS U6 WIP RNAi was a gift from Jack Strominger (Addgene plasmid # 11774 ; http://n2t.net/addgene:11774 ; RRID:Addgene_11774)

  • For your References section:

    Formation of a WIP-, WASp-, actin-, and myosin IIA-containing multiprotein complex in activated NK cells and its alteration by KIR inhibitory signaling. Krzewski K, Chen X, Orange JS, Strominger JL. J Cell Biol. 2006 Apr 10. 173(1):121-32. 10.1083/jcb.200509076 PubMed 16606694
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