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Addgene

GFP-MAPPER
(Plasmid #117721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4758
  • Total vector size (bp) 5517
  • Modifications to backbone
    ER signal peptide inserted upstream of GFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPPER
  • Species
    Synthetic
  • Insert Size (bp)
    759
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-MAPPER was a gift from Jen Liou (Addgene plasmid # 117721 ; http://n2t.net/addgene:117721 ; RRID:Addgene_117721)

  • For your References section:

    Feedback regulation of receptor-induced Ca2+ signaling mediated by E-Syt1 and Nir2 at endoplasmic reticulum-plasma membrane junctions. Chang CL, Hsieh TS, Yang TT, Rothberg KG, Azizoglu DB, Volk E, Liao JC, Liou J. Cell Rep. 2013 Nov 14;5(3):813-25. doi: 10.1016/j.celrep.2013.09.038. Epub 2013 Oct 31. 10.1016/j.celrep.2013.09.038 PubMed 24183667
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