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Addgene

pCINeo-mCHMP5
(Plasmid #11771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chromatin modifying protein 5
  • Alt name
    CHMP5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    660
  • GenBank ID
    BC006947
  • Entrez Gene
    Chmp5 (a.k.a. 2210412K09Rik, AW545668)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo-mCHMP5 was a gift from Sankar Ghosh (Addgene plasmid # 11771 ; http://n2t.net/addgene:11771 ; RRID:Addgene_11771)

  • For your References section:

    CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis. Shim JH, Xiao C, Hayden MS, Lee KY, Trombetta ES, Pypaert M, Nara A, Yoshimori T, Wilm B, Erdjument-Bromage H, Tempst P, Hogan BL, Mellman I, Ghosh S. J Cell Biol. 2006 Mar 27. 172(7):1045-56. 10.1083/jcb.200509041 PubMed 16567502
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