phSOD1-I113T-N’His
(Plasmid #117703)

• Purpose: mutation on stability/solubility of SOD1
• Depositing Lab: Elizabeth Fisher
• Publication: Fisher Lab SOD1 plasmids (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOD1
• Species: H. sapiens (human)
• Insert Size (bp): 571
• Mutation: I113T
• Entrez Gene: SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)
• Promoter: T7
• Tag / Fusion Protein:
  • His tag with Thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The I113T mutation of SOD1 found in patients with ALS, first described by Rosen et al. in 1993 (https://www.nature.com/articles/362059a0) uses old nomenclature and describes a mutation at position 113, not counting the start codon, whereas according to new nomenclature, including the start codon, this mutation would be at position 114 (I114T). To be consistent with the field we have decided to use the original name I113T for this human SOD1 mutation.