pmSod1-WT-N’His
(Plasmid #117701)

• Purpose: Expression of WT mouse SOD1 with N- terminal His tag
• Depositing Lab: Elizabeth Fisher
• Publication: Fisher Lab SOD1 plasmids (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sod1
• Species: M. musculus (mouse)
• Insert Size (bp): 467
• Entrez Gene: Sod1 (a.k.a. B430204E11Rik, Cu/Zn-SOD, CuZnSOD, Ipo-1, Ipo1, SODC, Sod-1)
• Promoter: T7
• Tag / Fusion Protein:
  • His tag with Thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Peter Joyce: pcDNA 3.1 with mouse WT SOD1 insert
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry