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PurposeMammalian expression vector encoding WT ScCas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneOG3569
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Backbone manufacturerOxford Genetics
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStreptococcus canis Cas9
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SpeciesStreptococcus canis
- Promoter EF-1α
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF-1α Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pScCas9 was a gift from Joseph Jacobson (Addgene plasmid # 117700 ; http://n2t.net/addgene:117700 ; RRID:Addgene_117700) -
For your References section:
Minimal PAM specificity of a highly similar SpCas9 ortholog. Chatterjee P, Jakimo N, Jacobson JM. Sci Adv. 2018 Oct 24;4(10):eaau0766. doi: 10.1126/sciadv.aau0766. eCollection 2018 Oct. 10.1126/sciadv.aau0766 PubMed 30397647
