p416 25Q GPD
(Plasmid #1177)

• Depositing Lab: Susan Lindquist
• Publication: Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(4):1589-94.

Backbone

• Vector backbone: p416 GPD
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: htt 25Q
• Species: H. sapiens (human)
• Insert Size (bp): 250
• GenBank ID: NM_002111
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

N terminal region of Ht with polyQ repeat length of 25 fused to GFP.