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Addgene

pPTbsr
(Plasmid #117696)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPTbsr
  • Backbone size (bp) 4168
  • Modifications to backbone
    Exchange of the bleomycin resistance gene of pPha-T1 to Blasticidin-S deaminase gene
  • Vector type
    Vector for Phaeodactylum tricornutum
  • Promoter fcpB
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPTbsr was a gift from Peter Kroth (Addgene plasmid # 117696 ; http://n2t.net/addgene:117696 ; RRID:Addgene_117696)

  • For your References section:

    Blasticidin-S deaminase, a new selection marker for genetic transformation of the diatom Phaeodactylum tricornutum. Buck JM, Rio Bartulos C, Gruber A, Kroth PG. PeerJ. 2018 Nov 14;6:e5884. doi: 10.7717/peerj.5884. eCollection 2018. 10.7717/peerj.5884 PubMed 30488015
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