pYUB1062-GFP
(Plasmid #117695)

• Purpose: (Empty Backbone) Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis
• Depositing Lab: Elizabeth Fullam
• Publication: Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095. doi: 10.1039/c8ra06237d. Epub 2018 Sep 25.

Backbone

• Vector backbone: pYUB1062
• Backbone manufacturer: pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein
• Backbone size (bp): 5800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • GFP (C terminal on backbone)
  • His (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Mycobacterium smegmatis mc24517 strain is required for expression
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.

This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.

References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam